Coding

Part:BBa_K5317007:Design

Designed by: Jan Gelhoet   Group: iGEM24_Hannover   (2024-09-13)


Murine MTF-1 gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1125
    Illegal PstI site found at 370
    Illegal PstI site found at 702
    Illegal PstI site found at 1159
    Illegal PstI site found at 1226
    Illegal PstI site found at 1264
    Illegal PstI site found at 1681
    Illegal PstI site found at 2011
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1125
    Illegal PstI site found at 370
    Illegal PstI site found at 702
    Illegal PstI site found at 1159
    Illegal PstI site found at 1226
    Illegal PstI site found at 1264
    Illegal PstI site found at 1681
    Illegal PstI site found at 2011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1125
    Illegal BamHI site found at 560
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1125
    Illegal PstI site found at 370
    Illegal PstI site found at 702
    Illegal PstI site found at 1159
    Illegal PstI site found at 1226
    Illegal PstI site found at 1264
    Illegal PstI site found at 1681
    Illegal PstI site found at 2011
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1125
    Illegal PstI site found at 370
    Illegal PstI site found at 702
    Illegal PstI site found at 1159
    Illegal PstI site found at 1226
    Illegal PstI site found at 1264
    Illegal PstI site found at 1681
    Illegal PstI site found at 2011
    Illegal NgoMIV site found at 1939
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To decrease the length of the sequence, it was important to use cDNA as a template instead of genomic DNA so introns would already be excluded. Start and Stop Codon was needed fo a successful translation.


Source

The MTF-1 gene sequence was aquired from NIH3T3 cDNA via PCR using the primers:

References